Recognition imprinted chiral phases

The molecular imprinting strategy can be applied for the recognition of different kinds of templates from small organic molecules to biomacromolecules as proteins. Some examples of separations investigated with MIP monoliths in CEC and LC are shown in Table 2. The influence of the imprinted monolithic phase preparation procedure and of the separation conditions on the selectivity and chromatographic efficiency have been widely studied [154, 157, 161, 166, 167, 192]. The performance of imprinted monoliths as chromatographic stationary phase has also been compared to that of the traditional bulk polymer packed column [149, 160]. It was shown that the monolithic phases yielded faster analyses and improved chiral separations. 

Stationary phases with specific molecular recognition properties for D,L-enantiomers of peptides have been tailored using the molecular imprinting technique. A template molecule is added to suitable monomer(s), the system is polymerized, and the chiral template molecule is washed out [128]. 

Fu, Q. Sanbe, H. Kagawa, C. Kunimoto K. Jun H., Uniformly Sized Molecularly Imprinted Polymer for (S)-Nilvadipine. Comparison of Chiral Recognition Ability with HPLC Chiral Stationary Phases Based on a Protein, Anal. Chem. 2003, 75, 191-198... 

Molecular imprinting technology offers a unique opportunity to provide chiral stationary phases or sensing devices with predefined chiral recognition properties... 

One of the most important features of molecular imprinting is to provide a means for creating robust polymeric recognition materials with predetermined ligand selectivity [5-7]. Thus, the main application of MIPs turned out to be in the field of separation sciences, as stationary phases in (electro)chromatographic analysis and chiral separations and as selective sorbents in solid phase... 

Andersson, L.I. O Shannessy, D.J. Mosbach, K. Molecular recognition in synthetic polymers preparation of chiral stationary phases by molecular imprinting of amino acid amides. J. Chromatogr. 1990, 513, 167-179. 

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