Receptors photomicrograph

FIGURE 26-5 Immunohistochemical localization of type I corticosteroid receptor (mineralocorticoid receptor) in the rat hippocampus. (A) Mineralocorticoid immunoreactivity is concentrated in pyramidal cell fields of the cornu ammonis (CA2). (B) High-power photomicrograph shows that steroid-bound mineralocorticoid receptors are primarily localized to neuronal cell nuclei. (Courtesy of Dr James P. Herman, Department of Anatomy and Neurobiology, University of Kentucky.)... 

FIGURE 43-2 Photomicrograph of the human neuromuscular junction. In normal muscle, Ach receptors are associated with the terminal expansions of the junctional folds and the architecture of the postjunctional membrane follows closely the distribution of active zones in the presynaptic membrane, b, basal lamina I, infoldings m, mitochondria M, myocyte N, nerve terminal r, ribosomes s, synaptic space S, Schwann cell. Courtesy of A. Engel. 

Fig. 5A-C. CBi expression on GABAergic terminals in rat somatosensory cortex. CBi receptors [arrowheads] were detected with an antibody directed againsttheC terminusof rat CBi using pre-embedding immunogold with silver enhancement. The boutons are forming symmetric synapses [arrows), characteristic of cortical GABAergic axon terminals. CBi -positive terminals form synapses with pyramidal cell bodies (A), main apical dendrites (B), and fine-caliber dendrite branches [C).Scalebar = 0.5 pm. (Original photomicrograph provided byTamas Freund and Agnes Bodor)...

Fig. 10. CBi expression in emetic centers. CB, is prominently expressed in the ferret area postrema (AP, dorsal vagal complex (DMNX, and associated regions involved in emesis as detected with a C-terminal CB, receptor antibody. Particularly strong immunostaining is present in a restricted group of cells in the area postrema as well as diffusely through the dorsal motor nucleus of the vagus (notice the lack of staining of cell bodies in DMNX), and the medial nucleus of the solitary tract (SolM. fourth ventricle CC, central canal. Scale bar = 100 pm. (Original photomicrograph provided by Marja Van Sickle and Keith Sharkey)...

Fig. 70. Bright-field (A) and dark-field (B) photomicrographs of a cerebellar folium of rat cerebellum labelled with [ HJmuscimol to demonstrate the GABA receptors. Note the clustering of grains over the area corresponding to the glomeruli of the granule cell layer and the low level of autoradiographic grains over the molecular layer (m) and the Purkinje cell layer (arrows). W, white matter. Bar = 100 /im. Palacios et al. (1981a).

Fig. 71. Bright-field photomicrographs showing cellular distribution of mRNAs of al, a6, 2, yS3, y2 and S GABAa receptor subunits in the cerebellum of the rat. Arrows indicate examples of labelled stellate/basket cells arrowheads delineate Purkinje cells. Gr, granule cells Mol, molecular layer P, Purkinje cells. Scale bar = 50 //m. Laurie et al. (1992).

Fig.78. A. x28 and B. x42 power photomicrographs of autoradiographs of iodocyanopindol ( ICYP) binding to adrenergic beta receptors in rat cerebellar cortex illustrating the high density of silver grains in the molecular layer and patches of increased receptor density over the Purkinje cell layer, m, molecular layer g, granule cell layer. (C) shows the patches at x 40 magnification with dark-field illumination. Sutin and Minneman (1985).

Fig. 24. Patch and matrix striatal compartments are labeled with neurochemical markers. A) The patch compartment is labeled with 3H-naloxone binding to mu opiate receptors (white in the darkfield photomicrograph). B) The matrix compartment is labeled with calbindin-immunoreactivity, which labels spiny projection neurons that provide inputs to the substantia nigra pars reticulata. The correspondence between calbindin-poor zones (black arrows) and mu opiate binding sites (white arrows) is seen to occur in all regions of the striatum. Calbindin-immunoreactivity is relatively weak in the dorso-lateral striatum, which nonetheless contains opiate receptor patches.

Figure 4 Photomicrographs of the comparative distribution of [l25I] [Leu31,Pro34]PYY binding sites and Y1 receptor mRNA in at the level of the frontal cortex of the human brain. Adjacent coronal sections were incubated with either 35 pM [12>I] [Leu31,Pro34]PYY in the presence or absence of 1 pM pNPY and 35S-sense and 35S-antisense of the NPY Y1 receptor mRNA. See list of abbreviations for details of anatomical identification.

The purified receptor-carrier was then found to be a glycoprotein of molecular weight about 50000. Under the electron microscope, it was seen as rows of rosettes, at a density of about a dozen rosettes to the linear 0.1 fim. This was first illustrated by Changeux (1980) and a more recent photomicrograph will be seen in Fig. 2.1. The sequence of all the amino acids is known. Several residues of carbohydrate are bound to the protein which has a somewhat lipophilic character. More about this ACh receptor is presented in Section 12.6. 

---