Receptor ligands, deconvolution

Another technique of single-bead, direct deconvolution is based on on-bead screening. When the biological assay can be run in this format, each library component is tested as resin bound in an assay medium where a soluble receptor is present and the receptor/ligand, or inhibitor, interaction takes place at the surface of the bead. After the biological assay a few positive beads are identified, usually through colorimetric, fluorescent, or radiolabeled detection. They are removed from the assay medium and characterized analytically after washing off the receptor. This technique was first described by Lam et al. [78], has since been... 

Isothermal titration calorimetry (ITC) is almost the ultimate titration methodology in that this technique is based entirely upon titration of heat energy and then deconvolution of this information into equilibrium binding constant information. However, the real beauty of this technique is that it engages directly with the thermodynamics of receptor-ligand binding interactions. 

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