Receptor binding assays fundamentals

Radioreceptor assays were developed as an offshoot of receptor ligand binding procedures. Accordingly, in order to fully appreciate the potential, and limitations, of radioreceptor assays, the fundamental principles of receptor binding assays must first be understood. Since only those issues... 

If modifications in the receptor binding procedure are essential for developing a particular radioreceptor assay, then it is crucial that the receptor binding be redefined with regard to its kinetics and pharmacological specificity. Without such data, it is impossible to be certain that the appropriate receptor site is being labeled, which is a fundamental assumption for radioreceptor assays. 

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