Real-time PCR primer

Pattyn, F. Speleman, F. De Paepe, A. Vandesompele, J. RT Primer DB the Real-time PCR primer and probe database. Nucl. Acids Res. 2003, 31, 122-123. 

Animal species determination in processed material or food products can be performed by PCR amplification of mitochondrial DNA followed by sequencing analysis of the product (see Chapter 6) or by RFLP. RFLP analysis can be successful only if the DNA detected is from a species with a known RFLP pattern. In addition, if DNA from only one animal is detected by sequencing analysis or RFLP, there is actually no guarantee that DNA from other species is also present at very low amounts. Low contaminations can also be detected by real-time PCR. For sequencing analysis or RFLP, PCR primers are used that amplify DNA from almost aU mammals. In contrast, for real-time PCR, primers specific for each species have to be used. 

---