Real-time PCR analysis

Castiglione F, Degl Innocenti DR, Taddei A, et al. Real-time PCR analysis of RNA extracted from formalin-fixed and paraffin-embeded tissues effects of the fixation on outcome reliability. Appl. Immunohistochem. Mol. Morphol. 2007 15 338-342. 

In contrast to RFLP analysis and standard PCR techniques, real-time PCR analysis can easily and rapidly detect a single nucleotide polymorphism (SNP) in a gene of interest without the need to run... 

Fronhoffs 8, Pruning T, Ortiz-PaUardo E, Erode P, Koch B, Harth V, et al. Real-time PCR analysis of the N-acetyltransferase NATl aUele 3, M, 10, 11, 14 and " 17 polymorphism in squamous cell cancer of head and neck. Carcinogenesis 2001 22 1405-12. 

Belgrader, P., S. Young, B. Yuan, M. Primeau, LA. Christel, F. Pourahmadi, and M.A. Northrup. A battery-powered notebook thermal cycler for rapid multiplex real-time PCR analysis. Anal Chem. 2001, 73(2), 286-289... 

Noncontact heating utilizing a focused microwave source was demonstrated by Fermer et al. [141]. In their work, a single-mode microwave cavity was used to heat 100 pL of PCR mixture in a 0.5-mL polypropylene tube for 25 cycles. Most recently, microwave-induced milliliter-scale PCR (see Fig. 8) was reported [142] for real-time PCR analysis. Although the amount of amplified nucleic acid product... 

Selection of positive amplification according to real-time PCR analysis or gel/capillary electrophoresis. 

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