Reactants hydrogen ions, binding calculations

Since JVH and NMg can be calculated for these reactants, ArJVH and Ar/VMg can be calculated as a function of pH and pMg. However, when a computer is available there is an easier way to do this by using equation 1.3-13 for the binding of hydrogen ions and 1.3-14 for the binding of magnesium ions. For example, equation 1.5-1 can be written... 

Calculation of the Binding of Hydrogen Ions by Reactants and the Changes in Binding of Hydrogen Ions in Biochemical Reactions... 

CALCULATION OF THE BINDING OF HYDROGEN IONS BY REACTANTS AND THE CHANGES IN BINDING OF HYDROGEN IONS IN BIOCHEMICAL REACTIONS... 

These tables can be used to calculate ArG ° and ATH ° at pH 7 and ionic strengths of 0, 0.10, and 0.25 M or at ionic strength 0.25 M and pHs of 5, 6, 7, 8, and 9 for any reaction for which all the reactants are in these tables. They can also be used to calculate standard apparent reduction potentials. The species data can be used to calculate average bindings of hydrogen ions by reactants. Mathematica programs for carrying out these calculations are provided. 

One of the Maxwell relations in equation 3.4-14 shows that the average binding of hydrogen ions by a reactant can be calculated by taking the partial derivative of the standard transformed Gibbs energy of formation of a reactant with respect to pH. 

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