Rapid screening of novel dehydrogenases

Even when highly rehable computer modeling techniques exist for dehydrogenases, the need for rapid screening of dehydrogenases will remain, both to verify the predictions experimentally and to determine basic kinetic parameters (substrate 

Km and values) and stereochemical properties. Ideally, screening should he carried out under reaction conditions that mimic the final process as closely as possible. This step is often one of the most time-consuming phases of process development, and improvements here can have significant impacts. 

Traditionally, libraries of enzymes - either naturally occurring or deliberately created mutants - are created in bacterial cells and the resulting clones are screened (Fig. 2, left). This is a time-tested strategy, but it suffers from two 

Dehydrogenase modules within larger assemblies as monofunctional catalysts 

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