Raman imaging microscope

Gift, A. D., Ma, J., Haber, K. S., McClain, B. L., and Ben-Amotz, D. (1999), Near-infrared Raman imaging microscope based on fiber-bundle image compression, J. Raman Spectrosc., 30,757-765. 

Figure 1.22 Schematic diagram of the Raman imaging microscope reported by Goldstein et al.. Reproduced with permission from Ref [63],...

A fast NIR Raman imaging microscope system (NIRIM) is described by McLain and coauthors that uses a fiber-optic bundle and CCD detector to collect a complete 3D Raman data cnbe from a sample in 1 s or less. A schematic of the NIRIM is shown in Figure 4.81. The system has been nsed... 

Raman spectroscopy is now coming of age as a routine analytical method. Advances in Raman technology have meant that robust, user-friendly equipment can be manufactured at a reasonable cost. These advances are described, together with applications of Raman imaging microscope systems to polymer analysis. 11 refs. 

Hayazawa, N., Inouye, Y, Sekkat, Z., and Kawata, S. 2002. Near-held Raman imaging of organic molecules by an apertureless metallic probe scanning optical microscope. J. Chem. Phys. 117 1296-1301. 

Since its development by Delhaye and Dhamelincourt in 1975 [1] the epi-illumination Raman microprobe has become one of the most important input systems in Raman spectroscopy and is the instrument around which most Raman imaging systems are constructed. Epi-illumination instruments are almost always constructed around research-grade commercially available fluorescence microscope frames, with input optics modified to accept an exciting laser and with output optics modified to direct backscattered Raman signal to a spectrograph. 

A third class of sampling geometries involves Raman microscopy and closely related Raman imaging techniques. Combination of a Raman spectrometer with a modified optical microscope permits spectra to be obtained from very small sample regions, down to less than 1 pm laterally and a few microns in depth (Fig. 6.3). Raman microspectroscopy is a term generally used to describe this spatially resolved technique in which spectra are obtained... 

Figure 1.17 (a) Visual image of a 1 im-diameter polystyrene bead. The lower images show Raman images of the bead, recorded with a lOOx (NA = 0.95) infinity-corrected microscope objective at increments of (left to right) 1.0, 0.5 and 0.1 pm per step, respectively (b) Raman spectrum measured from the center of one of these beads. Reproduced with permission from Ref [41]. 

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