Rabbits global ischemia

The protective effects of the Mn(ii)-based SOD mimics against myocardial ischemia/reperfusion injury in the isolated rabbit heart and monkey heart have been investigated. Kilgore et subjected Langendorff perfused, isolated rabbit hearts to a 30 minute period of global ischemia followed by a 45 minute period of reperfusion. Upon reperfusion, an increase in left ventricular end-diastolic pressure occurred, which was attenuated when the hearts were perfused with 20/.iM SC-52608. Perfusion of SC-52608 also inhibited the release of creatine kinase and intracellular potassium, and reduced the extent of antibody binding to the intracellular protein myosin which occurs upon reperfusion of the ischemic heart. These studies indicated that SC-52608 is cardioprotective to the reperfused, ischemic isolated rabbit heart. 

A promising application of NMR is in the development and evaluation of procedures to afford protection to the heart during ischemia. For example, an early study (Hollis et al, 1977) compared the effects of total, global ischemia on the biochemical state and functional recovery of rabbit hearts under conditions of KCl arrest and reduced temperatures with those at reduced temperatures in the absence of KCl arrest. Typical results are shown in Figure 21 where the pH, ATP level, and PCr level of two identical rabbit hearts are compared following 40 min of total, global ischemia. One heart was arrested with 30 mM KCl solution prior to ischemia the other heart was made ischemic without prior arrest. The pH has decreased from the normal value of 7.18 to 7.0 in the case of the KCl-arrested heart, but it has decreased to 6.1 in the nonarrested heart. In addition, the ATP level is maintained at the control value in the KCl-arrested heart, while it is mark-... 

Figure21. Comparison ofnhe NMR spectra of KCl arrested and nonarrested rabbit hearts after 40 min of global ischemia. From Hollis et al (1978).

Boulu, R. G., Gueniau, C., Plotkine, M., Sofeir, M., 1981 Recovery from global cerebral ischemia in rabbits Influence of indomethacin. Eur. Neurol. 20, 230-234. 

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