Quinolones liquid chromatographic methods

Following their extraction and cleanup, residues of quinolone antibiotics in sample extracts can be determined by either direct nonchromatographic methods, or gas or liquid chromatographic methods. Spectrophotometric, fluorometric, or mass spectrometric detection systems have all been successfully used in quinolone analysis (Table 29.6). 

Confirmation of the identity of the suspected liquid chromatographic peaks in quinolone analysis can be made by converting the analytes to the corresponding decarboxylated derivatives and analyzing them by gas chromatography-mass spectrometry (195, 196). Most promising screening and even confirmatory methods in terms of simplicity, selectivity, and sensitivity, are those described by Munns et al. (196), Roybal et al. (188), and Eng et al. (193). 

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