Quenching gold nanoparticles

Dulkeith, E., Ringler, M., Klar, T. A., Feldmann, J., Javier, A. M. and Parak, W. J. (2005). Gold nanoparticles quench fluorescence by phase induced radiative rate suppression. Nano Lett. 5 585-589. 

Schneider, G., Decher, G., Nerambourg, N., Praho, R., Werts, M. H. V. and Blanchard-Desce, M. (2006). Distance-dependent fluorescence quenching on gold nanoparticles ensheathed with layer-by-layer assembled polyelectrolytes. Nano Lett. 6 530-536. 

Figure 20.6 Schematic diagram of the DNA detection using fluorescence quenching by gold nanoparticles. Oligonucleotide molecules are conjugated to gold surface vial Au-S bonds and the fluorescein molecules at the other end of the oligo sequence is adsorbed onto gold nanoparticles to form a constrained conformation.

Gold nanoparticles, both spheres or rods, quench collagen fluorescence by photonic absorption of the emission light of the fluorophore by the nanoparticles. The addition of Au nanospheres quenches the collagen fluorescence (Figure 20.13A) but it does not affect the absorption spectra of collagen (Figure 20.13B). 

When cells are mixed with gold nanoparticle suspension, the nanoparticles are located in the vicinity of cells in the extracellular region. Figure 20.15 shows a comparison of the protein fluorescence spectra of whole cells with and without spherical gold nanoparticles. Obviously gold nanoparticles quench the fluorescence of cells at their protein bands when excited at protein absorption wavelength of 280 nm. There are no obvious differoices between normal cells and cancer cells. 

Ghosh, S. K., Pal, A., Kundu, S., Nath, S., and Pal, T. (2004). Fluorescence quenching of 1-methylaminopyrene near gold nanoparticles size regime dependence of the small metallic particles. Chem. Phys. 395 366-372. 

Dulkeith, E., Morteani, A. C., Niedereichholz, T., Klar, T. A., and Feldmann, J. (2002). Fluorescence quenching of dye molecules near gold nanoparticles radiative and nonradiative effects. Phys. Rev. Lett. 89 203002-203004. 

Peng, Z., Chen, Z., Jiang, J., Zhang, X., Shen, G., and Yu, R. (2007). A novel immunoassay based on the dissociation of immunocomplex and fluorescence quenching by gold nanoparticles. Anal Chim. Acta. 583 40-44. 

Kato, N., and Caruso, F. (2005). Homogeneous, competitive fluorescence quenching immunoassay based on gold nanoparticle/polyelectrolyte coated latex particles. J. Phys. Chem. B 109 19604-19612. 

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