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Quantitative Reaction Phenotyping Expressed or Purified Enzyme Systems

6 Quantitative Reaction Phenotyping Expressed or Purified Enzyme Systems [Pg.497]

FIGURE 15.5 Definitive cytochrome P450 reaction phenotyping for A-demethylation of sildenafil - (a) Use of selective enzyme inhibitors (b) Use of recombinant enzymes. Reprinted from Hyland et al. (2001) by permission of Blackwell Publishing. [Pg.498]



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Enzyme expression

Enzyme systems

Enzymes expressed

Enzymes purified

Expression systems

Phenotype

Phenotype/phenotyping

Phenotypic

Phenotyping

Purified

Purified Enzyme Systems

Purifying

Quantitative expression

Quantitative reactions

Reaction expression

Reaction phenotyping

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