Quantitative Determination of Fluorescing Spots

Quantitative Determination of Fluorescing Spots a) Direct Evaluation 

Fluorescing spots can be evaluated directly on the adsorbent layer. The conditions for this are favourable The relation F 8 x f holds, where F = the fluorescence value, read from a suitable detector, 8 = radiation density of a fluorescent spot, regarded as a light source and / = spot area. The total fluorescence of a particular amount of substance is thus not significantly influenced by the spot shape. 

A linear relation between the surface under the fluorescence intensity curve and the amount of substance chromatographed has been found within a useful measuring range, using the PMQ II spectrophotometer (Firm 155) [351, 626] the calibration curves using a modified Aminco-microphotometer (Firm 4) were not linear [793]. 

Chromoscan-densitometer with accessory for evaluation of thin layers (Firm 78). 

Turner fluorometer model 111 with adaptation for TLC (Firm 33) cf also Camag-information QTL-65 and QDC-6-65 (Firm 33). 

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