Quantitative Analysis in Biological Matrices

The explanation of this result is illustrated in Fig. 1.37, which shows selected reaction-monitoring traces of the sample at 10 ng mL f It becomes obvious that the response ratio between the analyte and the IS has dramatically changed. On one side there is enhancement of the analyte s response and on the other side suppression of the internal standard (IS) signal. These effects are mainly caused 

LC-MS/MS has dramatically changed the way bionalysis is conducted. Accurate and precise quantitation in the pg ml scale is nowadays possible however one has to be aware of certain issues which are specific to mass spectrometric detection such as matrix effects and metabolite crosstalk. With the current growing interest in the analysis of endogenous biomarkers in biological matrices, quantitative bioanalysis with MS has certainly the potential to contribute further in this field with the development of multicomponent assays. Modern triple quadrupole instruments have the feature to use very short dwell times (5-10 ms), allowing the simultaneous determination of more than 100 analytes within the timescale of an HPLC peak. Due to the selectivity of the MS detection the various analytes 

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