Quantification SILAC

Figure 7 Protocol for quantification of proteins by the SILAC method. Reproduced from C. Dass, Fundamentals of Contemporary Mass Spectrometry, Wiley-lnterscience Hoboken, NJ, 2007, with permission from Wiley-lnterscience, Copyright 2007.

Quantification Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)... 

FIGURE 3.37 SILAC methodology for in vivo, relative protein quantification. 

The technique of isobaric tag for relative and absolute quantification (iTRAQ) is based on covalently labeling the amines at N-termini and the side chains of peptides with isotope-coded tags after the proteins have been isolated form biological systems, denatured, and enzymatically digested. Here the isotope labeling is carried out at a much later stage than in both SILAC and ICAT. iTRAQ is an MS/MS technique that uses CID-derived ions for both the identification and quantification of proteins. 

Figure 8.11. Protocol for quantification of proteins by the SILAC method.

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