Pyruvic quinoxalone derivatives

The quinoxalone derivatives of pyruvate and 2-ketobutyrate were separated on a /iBondapak Qg reversed-phase column (3.9 mm x 300 mm). The mobile phase was a mixture of acetonitrile, methanol, and 40 mM phosphate buffer (pH 7) (5 8 12, v/v/v) run at a flow rate of 1 mL/min. Detection could be carried out spectrophotometrically at 254 nm, or fluorometrically with excitation and emission wavelengths of 340 and 389 nm, respectively. 

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