Purification of the Light Chains

The key step in LC isolation is the preparation of pure smooth muscle myosin (Hasegawa et al., 1988). This is usually executed by homogenizing the muscle mince with salt solutions (60-100 mM ionic strength) to remove the cytoplasmic proteins and to a certain extent the thin filament proteins. A crude actomyosin is then extracted form the well-washed muscle residue with salt solutions containing ATP and the actin component is removed by ultracentrifugation. The LCs are 

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FIGURE 1 Autoradiograms of two-dimensional phosphopeptide maps of LC20 phospho-rylated in aortic homogenate. Six phosphopeptides, labeled A through F, are resolved. From Erdodi et al. (1988b, Hg. 2, p. 159). 

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