Purification of horseradish peroxidase

The rather expensive POase is usually purchased rather than puri- 

Purification of peroxidase by a single step on DEAE-Sepharose  

Prepara- tion Frac- tion Concentration of elution buffer (mM) RZ Recovery in % at % of total activity recovered Specific activity (%) 

Total recovery for crude POase 97% for pure POase 97.9%. 

A-Sepharose column followed by gel filtration. This method, however, is not recommended, since it is time consuming, expensive, applicable to small amounts (which become diluted) and does not remove the less active isozymes (Table 10.5). 

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Keilin D, Hartree EF (1951) Purification of horseradish peroxidase and comparison of its properties with those of catalase and methaemoglobin. Biochem J 49 88-106... 

Purification of glycosulphatase Purification of horseradish peroxidase Purification of human-liver p-D-galactosidasei Purification of P-D-2-acetamido-2-deoxy-hexosidases A and B from human placenta Purification of pepsin glycopeptides from human immunoglobulin IgAj myeloma glycoprotein... 

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