Pseudomonas gladioli

More recently the biotransformation of limonene by another Pseudomonad strain, P. gladioli was reported [76,77]. P. gladioli was isolated by an enrichment culture technique from pine bark and sap using a mineral salts broth with limonene as the sole source of carbon. Fermentations were performed during 4-10 days in shake flasks at 25°C using a pH 6.5 mineral salts medium and 1.0% (+)-limonene. Major conversion products were identified as (+)-a-terpineol and (+)-perillic acid. This was the first time that the microbial conversion of limonene to (+)-a-terpineol was reported, see pathway 4. The conversion of limonene to a-terpineol was achieved with an enzyme, a-terpineol dehydratase (a TD), by the same group [78]. The enzyme, purified more than tenfold after cell-disruption of Pseudomonas gladioli, stereospecifically converted (4 )-(+)-limonene to (4/ )-(+)-a-terpineol or (4S)-(+)-limonene to (4S)-(+)-a-terpineol. a-Terpineol is widely distributed in nature and is one of the most commonly used perfume chemicals [27]. 

Table I Antifungal activity to Fusarium oxysporum f. sp. lagenariae and affinity to welsh onion of Pseudomonas gladioli...

Lageneria siceraria) by mix-cropping with welsh onion or Chinese chive inoculated with Pseudomonas gladioli M-2196 2 (field test)... 

Root systems were dipped in Pseudomonas gladioli M-2196 culture solution for 5 min. 

Ci8H320,4 472.442 Constit. of the repeating unit of the O-polysaccharide of Pseudomonas gladioli allicola strain 8494 (313). 

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