Proximity of Charged Residues

The challenge then is to devise experiments that will allow individual assessment of these factors, especially as they are interdependent and the potentials are very sensitive to small perturbations. The results of experiments designed to test factors (l)-(3) above will now be addressed in turn. 

7 A from the S ligand atom of Cys6. Such weak electrostatic effects could help tune the site to the observed E° (Equation 4). 

Altered surface charges influence E° in some proteins and not in others. This appears to be related both to the distance of the charge from the active site and to the influence of solvating water in masking the effects of the charge.For 

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