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Proton resonances, hemoglobin

Fig. 12. Assignments of the proton resonances of the C-2 protons of histidyl residues at p2 and pi46 of human normal adult hemoglobin 250-MHz aromatic proton resonances of deoxy-Hb A, deoxy-Hb Deer Lodge (p2His— Arg), and deoxy-des-His-(pi46-deleted)-Hb A in 0.1 M Bis—Tris in D20 at pH 6.3 and 27°C. The resonance lines are labeled according to the notation of Russu et al. (1982). [From Ho and Russu (1981)]. Fig. 12. Assignments of the proton resonances of the C-2 protons of histidyl residues at p2 and pi46 of human normal adult hemoglobin 250-MHz aromatic proton resonances of deoxy-Hb A, deoxy-Hb Deer Lodge (p2His— Arg), and deoxy-des-His-(pi46-deleted)-Hb A in 0.1 M Bis—Tris in D20 at pH 6.3 and 27°C. The resonance lines are labeled according to the notation of Russu et al. (1982). [From Ho and Russu (1981)].
Assignments of Proton Resonances of Human Normal Adult Hemoglobin ... [Pg.195]

Finally, there is another piece of experimental evidence supporting the suggestion that the ferrous hfs proton resonances are sensitive to the quaternary structural state of hemoglobin. In 1971, we investigated the ferrous hfs proton resonances of four mutant deoxyhemoglobins, Hb Chesapeake [a92(FG4)Arg —> Leu], Hb J Capetown [a92(FG4)Arg - Gin], Hb Yakima [ 399(Gl)Asp - His], and Hb Kempsey [099(Gl)Asp —> Asn], which have amino acid substitutions in the ai(J2 subunit interface (Davis etal., 1971) The locations of these mutant Hbs are shown in Fig. 11. Figure 39 shows the effects of these... [Pg.243]

Fig. 49. 600-MHz H NMR spectra of cross-linked mixed-valency asymmetric hybrid hemoglobin (a+CNp)A(aP)cXL, in deoxy and CO forms, in the presence of 0.1 Af phosphate at pH 6.8 and 21°C. (A) Hyperfine-shifted proton resonances in D20 (B) exchangeable and hyperfine-shifted proton resonances in H20. [From Miura and Ho (1982)]. Fig. 49. 600-MHz H NMR spectra of cross-linked mixed-valency asymmetric hybrid hemoglobin (a+CNp)A(aP)cXL, in deoxy and CO forms, in the presence of 0.1 Af phosphate at pH 6.8 and 21°C. (A) Hyperfine-shifted proton resonances in D20 (B) exchangeable and hyperfine-shifted proton resonances in H20. [From Miura and Ho (1982)].
PROTON NUCLEAR MAGNETIC RESONANCE STUDIES ON HEMOGLOBIN COOPERATIVE INTERACTIONS AND PARTIALLY LIGATED INTERMEDIATES... [Pg.153]

Proton Nuclear Magnetic Resonance Studies on Hemoglobin Cooperative Interactions and Partially Ligated Intermediates... [Pg.400]

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Proton resonance

Proton resonances, hemoglobin assignment

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