Proton magnetic resonance spectroscopy deuterium

Figure 4. Proton magnetic resonance spectroscopy of the anomeric proton region of peptidophosphomannan (10). Fifty mg of peptidophosphomannan was dissolved in H20 and held at room temperature for 1 hr. The solvent was removed under reduced pressure and the residue redissolved in 1 mL H20. The NMR spectrum was taken on a Varian XL-100 MHz spectrometer at 29°C with the instrument locked on deuterium and referenced to an internal standard of sodium 2,2-dimethyl-2-silapentane-5-sulfonate.

R. M. Smith, O. Chienthavorn, I. D. Wilson, and B. Wright, Superheated deuterium oxide reversed-phase chromatography coupled to proton nuclear magnetic resonance spectroscopy, Anal. Commun. 35 (1998), 261-263. 

Deuterium magnetic resonance spectroscopy has found wider applications since the advent of highly sensitive Fourier transform spectrometers. Deuterium is preferred choice in molecular and in H NMR spectroscopy in order to provide additional information about proton vibrations and resonances spectra measured before and after deuteration help to locate the exchanging hydrogen atoms. 

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