Protocols for Gel Analysis

The next sections will demonstrate various methods of analyses used for common gel types. 

The origin of defects causing discoloration in polyolefin pellets can be identified using light and electron microscopy. For example, PE pellets from an in-plant recycle re-pelletlzing process contained pellets that were off color and had black specks, as shown In Fig. 11.6(a). One of these defects was isolated using the cross sectioning technique, as shown in Fig. 11.6(b). The cross section revealed an intense reddish particle that caused the discoloration of the pellet. 

Once the contaminant is identified, the troubleshooter must determine how the material entered the feedstock stream. Process controls must be identified and implemented to mitigate the contaminant source. 

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Detailed Protocol for Gel Analysis of Randomly Amplified Polymorphic DNA Reactions... 

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