Protoaminobacter rubrum

Enzymatic rearrangement of sucrose with Protoaminobacter rubrum CBS 574.77 performed on an industrial scale, affords isomaltulose (also named palatinose ) widely applied as artificial sweetener. Interesting optically pure derivatives (e.g. spiro-system f or analogs of nojirimycin 4) were obtained by hydrolysis of the glycosidic bond in functionalized sucroses. Sucralose (l, 4,6 -trideo)gr-l, 4,6 -trichloro-gaZarto-sucrose), prepared by chlorination of 6-0-acetylsucrose with S02Cl2/pyridine, represents an example in which the disaccharide skeleton is only slightly modified. 

It is produced by running 40-60% solutions of pure sucrose through a reactor equipped with immobilized cells of Protoaminobacter rubrum. Other important microorganisms that are capable of this transformation are Leuconostoc mesenter-oides, Serratia plymuthica, Serratia marcescens, Erwinia carotovora, Erwinia rhapontici. 

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