Proteomics using bioinformatics

The most common implementation of proteomic analysis involves protein separation two-dimensional gel electrophoresis (2DGE), quantification of proteins with analytical methods for their identification in mass spectrometer (MS), and at the very least data integration and analysis using bioinformatic tools. 

Juan HF, Lin JYC, Chang WH, Wu CY, Pan TL, Tseng MJ, Khoo KH, Chen ST. Biomic study of human meyloid leukemia cells differentiation to macrophages using DNA array, proteomic, and bioinformatic analytical methods. Electrophoresis 2002 23 (15) 2490-504. 

Further, the dedicated analytical approaches to characterize the pool of metal-containing species in living organisms, the metallome, and of their interactions with the genome, transcriptome, proteome and metabolome were grouped into in vivo detection, localization, identification and quantification, in vitro functional analysis and in silico prediction using bioinformatics, which can be illustrated as in Figure 1.9. 

Proteomics is an interdisciplinary science that includes biology, bioinformatics, and protein chemistry. The purpose of this book is to provide the reader with an overview of the types of questions being addressed in proteomics studies and the technologies used to address those questions. The first chapter is a concise outline of the field as it presently stands. The second chapter provides an overview of the use of 2D-gel electrophoresis and mass spectrometry to identify proteins, as well as post-translational... 

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