Proteomics Immunoaffinity purification

Vesilescu, J., J. C. Smith, M. Ethier, and D. Figeys. 2005. Proteomic analysis of Ubiquitinated proteins from MCF-7 breast cancer cells by immunoaffinity purification and mass spectrometry. J. Proteome. Res. 4, 2192-2200. 

The use of immunoaffinity purification as a sample preparation method for downstream proteomics applications is emerging for applications involving identification of proteins in functional complexes and global identification of modified proteins. This approach allows investigators to use proteomics approaches to address hypothesis-driven research questions. Although a detailed protocol was presented here, it should be noted that each sample type and antibody will require optimization of the protocol—different buffers may work better for lysis, antibodies may bind more or less... 

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