Proteins secondary and tertiary structure

It is commonly agreed that enzymes are inactive in nearly anhydrous dimethyl sulfoxide (DMSO) [55] and that such inactivity might be a direct result of protein solubilization in the organic milieu, which causes deleterious changes in the proteins secondary and tertiary structures [56]. However, different authors have recently reported that some proteases, namely thermolysin (from Bacillus thermo-proteolyticus) and Proleather (from Bacillus subtilis), were still active in pure DMSO, despite the fact that proteins were indeed solubilized. The former enzyme cata-... 

Meiler J, Baker D. Coupled prediction of protein secondary and tertiary structure. Proc Natl Acad Sci USA 2003 100 12105-12110. 

The advent of recombinant DNA technology has led to an increased interest in the structural characterization of proteins by spectroscopic methods. Few spectroscopic techniques can provide the amount of information regarding protein secondary and tertiary structure which can be obtained from circular dichroism (CD) spectroscopy. In this chapter we describe the capabilities of CD spectroscopy to provide details on the globular structure of proteins. In addition, we will provide an overview of quantitative secondary structure estimates via CD spectroscopy and of specialized CD methods for studying proteins in contact with membranes and other biomolecules. Certain aspects of protein CD spectroscopy have been previously reviewed [1-19]. 

Primary, Secondary, Tertiary, and Quaternary Structure of Proteins Secondary and Tertiary Structures of Nucleic Acids... 

Many important systems and processes, especially biological assemblies such as cell walls and protein secondary and tertiary structures form as a result of physical, inter- and intramolecular interactions. Such assemblies and... 

ELECTROSTATIC PROPERTIES OF PROTEINS ROLE OF THE AQUEOUS SOLUTION IN DETERMINING PROTEIN SECONDARY AND TERTIARY STRUCTURE... 

---