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Proteins Sanger degradation

By the primary structure of a peptide or protein, we mean its amino acid sequence. Complete hydrolysis gives the amino acid content. The N-terminal amino acid can be identified by the Sanger method, using 2,4-dinitrofluorobenzene. The Edman degradation uses phenyl isothiocyanate to clip off one amino acid at a time from the N-terminus. Other reagents selectively cleave peptide chains at certain amino acid links. A... [Pg.317]

The structure and properties of peptides and proteins depend critically upon the sequence of amino acids in the peptide chain. The first complete amino acid sequence of a protein, that of insulin (51 amino acid residues), was determined by F. Sanger in 1953. The process is now performed using automated protein sequencers, and involves step-by-step identification of amino acids at the N terminus of the protein using a chemical process known as Edman degradation. [Pg.78]

Alkalies have not been used extensively for degradation of proteins and polypeptides. Their limitations have been recognized for some time, and few attempts have been made in recent years to further evaluate this medium for hydrolysis of proteins. Much of the available information on alkaline hydrolysis has been reviewed elsewhere (Sanger, 1952 Leach, 1953 Desnulle, 1953), and only a few comments are necessary here. [Pg.61]

Mixtures of peptides result from partial degradation of proteins. Separation of the individual components and establishment of their amino acid sequence form the basis for elucidating the chemical structure of proteins for information about the methods of degradation which yield larger and smaller peptides, reference may be made to Sanger [130] (partial hydrolysis) Craig et al. (oxidative fission of S—S... [Pg.752]


See other pages where Proteins Sanger degradation is mentioned: [Pg.1196]    [Pg.119]    [Pg.160]    [Pg.475]    [Pg.374]    [Pg.17]    [Pg.184]    [Pg.91]    [Pg.94]    [Pg.117]    [Pg.625]    [Pg.93]    [Pg.625]    [Pg.177]    [Pg.342]    [Pg.205]    [Pg.343]    [Pg.103]    [Pg.178]    [Pg.3917]    [Pg.3918]    [Pg.555]    [Pg.155]    [Pg.6828]    [Pg.13]    [Pg.625]    [Pg.125]    [Pg.151]    [Pg.625]   
See also in sourсe #XX -- [ Pg.1202 ]




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