Proteins, large labelling technique advances

Protein NMR has advanced with the help of stable isotope labeling techniques, which allowed to obtain multinuclear multidimensional spectra. To apply the NMR for large proteins, the control of relaxation time is also important.21,120 For instance, a deuteration for non-exchangeable protons significantly attenuates the relaxation rates. In this case, the amount of NOEs also reduced depending on the deuteration. Therefore, other complementary NMR-derived information will be required (Section 9). 

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