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Proteins ion exchange chromatography

As shown in the purification of rat histone H2A1 53 amide synthesized using the FMOC strategy, remarkable results using a Pharmacia Biotech Mono S column were obtained for the purification of complex synthetic mixtures (Figures 3 and 4)J5H] This system has been used with similar success with several other peptides. With a complex mixture of peptide/proteins, ion-exchange chromatography was found to be an important first step for preliminary fractionation to be further purified by RP-HPLC. [Pg.643]

Many chemically different stationary phases are used in liquid chromatography of proteins. Ion exchange chromatography uses an ion exchange resin, and the proteins are eluted with buffer solutions differing in ionic strength... [Pg.37]

See other pages where Proteins ion exchange chromatography is mentioned: [Pg.460]   
See also in sourсe #XX -- [ Pg.74 , Pg.75 , Pg.76 , Pg.77 , Pg.78 , Pg.263 ]

See also in sourсe #XX -- [ Pg.840 ]



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