Protein gel electrophoresis

Breci, L., Hattrup, E., Keeler, M., Letarte, J., Johnson, R., Haynes, PA. (2005). Comprehensive proteomics in yeast using chromatographic fractionation, gas phase fractionation, protein gel electrophoresis, and isoelectric focusing. Proteomics 5, 2018-2028. 

O Shaughnessy RF et al. PA-FABP, a novel marker of human epidermal transit amplifying cells revealed by 2D protein gel electrophoresis and cDNA array hybridisation. FEBS Lett 2000 486 149-154. 

L. Breci, E. Hattrup, M. Keeler, J. Letarte, R. Johnson, and P. A. Haynes. Comprehensive Proteomics in Yeast Using Chromatographic Fractionation, Gas Phase Fractionation, Protein Gel Electrophoresis, and Isoelectric Focusing. Proteomics, 5(2005) 2018-2028. 

Patton, W. F. (1995). Biologist s perspective on analytical imaging systems as applied to protein gel electrophoresis.. Chromatogr. 698, 55-87. 

The principles of gel electrophoresis are explained in Chapter 2. Proteins are more complex than nucleic acids, in this respect, since the charge on a protein may be positive, negative, or zero, whereas nucleic acids are always negatively charged. Polyacrylamide is the gel of choice for protein gel electrophoresis. It is possible to electrophorese proteins in the absence of a gel, by doing the experiment in a very narrow tube, to reduce lateral diffusion. This technique is known as capillary gel electrophoresis and is gaining popularity for its resolution, speed, and reproducibility, although it requires complex equipment. 

Precast protein gel electrophoresis NuPAGE Novex 4-12% Bis-Tris Gel 1.0 mm, 15 well (Invitrogen). 

Therefore, unlike DNA gel electrophoresis, two kinds of protein gel electrophoresis are possible native gel electrophoresis, where the net charge and shape of the protein contribute to the migration properties of the molecules, and denaturing gel electrophoresis, where all the secondary interactions have been disrupted and an overall negative charge has been introduced to the molecule, thereby allowing for a size-based separation. The latter is commonly known as an SDS-PAGE (sodium... 

Therefore, the movement of the ions within the gel is directly proportional to the applied voltage, and inversely proportional to the size of the protein. Gel electrophoresis is run for a fixed amount of time, allowing the small proteins to migrate further than the larger ones, resulting in their separation based on their size. Coomassie blue or silver staining can be used to detect the bands across the gel to confirm the presence of proteins with known molecular masses. 

Simple tandem repeat loci are attractive alternatives, in my view, to protein gel electrophoresis for measuring polymorphism relevant to many population... 

A variety of parameters are used to characterize polypeptides and proteins. One of the most fundamental is molecular weight. Gel electrophoresis can be used to measure the approximate molecular weight of a protein. Gel electrophoresis involves migration of a peptide or protein dissolved in a buffer through a porous polymer gel under the influence... 

Protein gel electrophoresis system (Mini-Protean 3 Cell from Bio-Rad or equivalent). 

Von Eggehng, F. Gawriljuk, A. Fiedler, W Ernst, G. Claussen, U. Klose, J. Romer, I. Huorescent dual colour 3D-protein gel electrophoresis for rapid detection of differences in protein pattern with standard image analysis software. Int. J. Mol. Med. 2001,8, 373-377. 

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