Protein trans-splicing

Evans, T. C. Jr., Martin, D., Kolly, R., et al. (2000) Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803. /. Biol. Chem. 275, 9091-9094. 

Wu H, Hu ZM, Liu XQ. Protein trans-splicing by a split intein encoded in a spht dnae gene of synechocystis sp. Pcc6803. Proc. Natl. Acad. Sci. U.S.A. 1998 95(16) 9226-9231. 

Stm W, Yang J, Liu XQ. Synthetic two-piece and three-piece spht inteins for protein trans-splicing. J. Biol. Chem. 2004 279(34) 35281-35286. 

Shi J, Muir TW. Development of a tandem protein trans-splicing system based on native and engineered split inteins. J. Am. Chem. Soc. 2005 127(17) 6198-6206. 

Kwon Y, Coleman MA, Camarero JA. Selective immobilization of proteins onto solid supports through spUt-intein-mediated protein trans-splicing. Angew. Chem/ Int. Ed. Engl. 2006 45(11) 1726-1729. 

Dhar T, Mootz HD (2011) Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein. Chem Commun 47 3063-3065... 

R509 G. Volkmann and H. Iwai, Protein Trans-Splicing and Its Use in Structural Biology Opportunities and Limitations , Mol. BioSyst., 2010, 6, 2110. 

Ray, C., Abbott, A.G. and Hussey, R.S. (1994) Trans-splicing of a Meloidogyne incognita mRNA encoding a putative esophageal gland protein. Molecular and Biochemical Parasitology 68, 93—101. 

Fig. 1.5 A Natural protein splicing, B Trans splicing with a split intein. The two fragments can be prepared separately and reassembled in vitro to form an active intein domain for protein ligation. C Central segment labeling using two different split inteins.

A few cases are known in which proteins undergo trans splicing. For example, the dnaE gene of Synechocystis, which codes for DNA polymerase III,... 

Fig. 9.10. Ligation of two protein fragments by trans-splicing interns a) on the DNA level, the two fragments can be encoded in separate ORFs and are flanked by either a 3 -extension encoding the N-intein (N-fragment) or 5 -extension encoding the C-intein (C-fragment). b) Following transcription and translation, the intein domains dimerize and c) excise themselves, fusing the N- and C-fragment by a peptide backbone bond.

Vellard, M., Sureau, A., Soret, J., Martinerie, C. Perbal, B. (1992) Proc. Natl. Acad. Sci. USA 89, 2511 -2515. A potencial splicing factor is encoded by the oposite strand of the frara-spliced c- myb exon. Breen, M.A. Ashcroft, S.J.H. (1997) FEBSLett. 409, 375-379. A truncated isoform of Ca /calmod-ulin-dependent protein kinase 11 expressed in human islets of Langerhand may result from trans-splicing. 

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