Protein surfaces, hotspots

RRB and APEE mechanisms represent extreme cases. The RRB mechanism is to be favoured in cases in which strong protein adsorption is not apparent. The APEE mechanism, on the other hand, is to be favoured wherever diffusion-controlled voltammetry is observed despite strong adsorption with saturative electrode surface coverage. For a heterogeneous electrode surface, it is quite likely that both mechanisms can operate simultaneously with RRB and APEE occurring, respectively, at coolspots and hotspots . 

Figure 42 VRML scene of the p53 DNA complex. Both molecules are represented in wire frame (blue, DNA fragment yellow, c2 domain of the p53 protein red, mutation hotspots). The surface indicates the p53 protein DNA interface region. The surface is color coded according to the electrostatic potential calculated using the Poisson-Boltzmann equation (see Figure 34) (green, negative gray, neutral red. positive)...

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