Protein stoichiometry

Kenakin, T. P. (1997). Differences between natural and recombinant G-protein coupled receptor systems with varying receptor/G-protein stoichiometry. Trends Pharmacol. Sci. 18 456-464. 

Lottspeich, F. (2005). Protein stoichiometry of a multiprotein complex, the human sphceosomal U1 small nuclear ribonudeoprotein absolute quantification using isotope-coded tags and mass spectrometry. /. Biol. Chem. 280, 2536-2542. 

The metal to protein stoichiometry was determined by atomic absorption spectroscopy using an Instrumentation Laboratory IL157 spectrometer. The concentration of metal ions in the RAGl proteins was measured in solutions of 3 to 10 pM protein. These were compared to either a Zn or Cd calibration curve ranging from 1 to 15 pM, which was measured prior to each protein sample. 

To determine the metal-to-protein stoichiometries of the zincbinding domains of RAGl atomic absorption spectroscopy was used. As expected, MBP-RF, which contains only the RING finger sequence... 

Enami I, Kaneko M, Kitamuta N et al. Total immobilization of the extrinsic 33 kDa protein in spinach Photosystem II preparations. Protein stoichiometry and stabilization of oxygen evolution. Biochim Biophys Acta 1991 1060 224-232. 

L3mch JB, Juarezgarcia C, Miinck E, Que Jr L. 1989. Mossbauer and EPR smdies of the binuclear iron center in ribonucleotide reductase from Escherichia colt a new iron-to-protein stoichiometry. J Biol Chem 264 8091-8096. 

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