Protein Removal for Optimum Sensitivity

Data Reduction and Automated Interpretation of CPC Spin Column/ESI-MS Data 

The interpretation of the large amounts of data generated in a screening campaign cannot be performed manually. Customized software has been designed to automatically evaluate the data and search for the compounds that non-covalently bind to the protein [21, 22]. For flow injection ESI-MS data of GPC spin column eluates of ten component mixtures, the following steps were taken to automatically interpret the data. The raw data were combined, smoothed, background 

Uoad FotieQround emd Background Spectra into the Program 

Calculate the Inter sUy Ratio between the Foreground and Background Peaks ot the Same Nominal Mass 

TIC - 3.38B404 BG Ratio - 1.666404 Base Peak - 284.1 (1.7S 403 a ss Mstx9)ad Icn Intensity Abtndaftoe isotcije Fit 

---