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Protein optical assay

The Soy Protein Residue Assay is a double-antibody (sandwich) ELISA using specific anti-soy tripsyn inhibitor and other soy protein antibodies coated onto microwells. After addition of the sample, the enzyme conjugate, and the TMB substrate, a positive reaction (indicating the presence of soy protein), produces a blue color. Addition of the stop solution ends the assay and turns blue to yellow. The result may be read visually (in the qualitative method) or with an ELISA reader (in the qualitative or quantitative method). Quantification can be obtained by mnning positive control standards (2.5-5-10-25 ppm) together with the samples. A standard curve is then plotted using the optical density (OD) values of the control standards (OD vs. concentration). [Pg.341]

Fig. 2 2D DQ/SQ NMR correlation of the fully hydrated N]-Leu-BR sample using a Bruker AV-III 600 MHz wide-bore spectrometer with an MAS rate of 8 kHz (a) and the protein function assay through detection of the M state signal and proton pumping cycle signal at 412nm (b) and 456nm (c) by optical dynamic spectroscopy, respectively... Fig. 2 2D DQ/SQ NMR correlation of the fully hydrated N]-Leu-BR sample using a Bruker AV-III 600 MHz wide-bore spectrometer with an MAS rate of 8 kHz (a) and the protein function assay through detection of the M state signal and proton pumping cycle signal at 412nm (b) and 456nm (c) by optical dynamic spectroscopy, respectively...
Experiments in 500 ml Erlenmeyer flasks and Fernbach flasks contained 200 ml and 1 L of EPl and EP2 medium respectively. Inocuia added to these cultures was 2 ml of spore suspension (5.0 optical density at 540 nm) for each 100 ml EP medium. All cultures were grown at 37°C in a shaking incubator (New Brunswik Sci. Co., USA), at 200 rpm. Then 10 ml of sample were withdrawn each 24 h during fermentation and immediately filtered through Millipore membranes of 0.45 pm pore size these cell-free filtrates were used for enzymatic assays and extracellular protein determinations by the Lowry method (14). Experiments in the 14 L fermentor (Microgen Fermentor New Brunswik Sci. Co., USA) were carried with lOL of fermentation medium EP2 and inoculum added was IL of mycelium grown 24 h in... [Pg.894]

Fig. 12. Diagram of elution pattern of red cell acid phosphatase and various markers on Biogel P 60. The position of the various protein markers was determined both by optical density determination and by starch gel electrophoresis of the individual fractions (83). The experiment was carried out using a polyacrylamide gel (Biogel P 60, 50-150 mesh exclusion limit >60,000 Bio-Rad Laboratories, California) in 0.05 M tris buffer, pH 8.0, containing 0.08% (v/v) Tween 80 and 0.1% (v/v) 2-mercaptoethanol to stabilize the enzyme. Column 60 X 4 cm. Flow rate 20 ml/hr, 4 ml fractions. (A) OD at 280 nm, ( ) OD at 540 nm, ( ) LDH assay with p-nitrophenyl phosphate for AcP. From Hopkinson and Harris (85). Fig. 12. Diagram of elution pattern of red cell acid phosphatase and various markers on Biogel P 60. The position of the various protein markers was determined both by optical density determination and by starch gel electrophoresis of the individual fractions (83). The experiment was carried out using a polyacrylamide gel (Biogel P 60, 50-150 mesh exclusion limit >60,000 Bio-Rad Laboratories, California) in 0.05 M tris buffer, pH 8.0, containing 0.08% (v/v) Tween 80 and 0.1% (v/v) 2-mercaptoethanol to stabilize the enzyme. Column 60 X 4 cm. Flow rate 20 ml/hr, 4 ml fractions. (A) OD at 280 nm, ( ) OD at 540 nm, ( ) LDH assay with p-nitrophenyl phosphate for AcP. From Hopkinson and Harris (85).
Fia. 5. Reaction of E. coli pyrophosphatase with TNBS at pH 8.5 in 0.5 M NaHCOs. The reaction was followed in a spectrophotometer, and the concentrations of e-TNP-lysine were determined from the optical density at 345 m/i. The solid line shows these data as percentage of total protein lysines by 6 hr the optical density had stabilized at the level indicated by the arrow on the right-hand ordinate. Aliquots were removed at the times shown (circles) and assayed for remaining enzymic activity. [Pg.515]

G. Cacciatore, M. Petz, S. Rachid, R. Hakenbeck and A. Bergwerff, Development of an optical biosensor assay for detection of /Mactam antibiotics in milk using the penicillin-binding protein 2 x, Anal. Chim. Acta, 520 (2004) 105-115. [Pg.491]


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See also in sourсe #XX -- [ Pg.398 , Pg.399 ]




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