Protein Identification Resource

ATLAS of Protein and Genomic Sequences Protein Identification Resource (PIR)... 

An important development in high-throughput protein identifieation is the introduction of protein database searching [111]. After separation on ID- or 2D-GE, the proteins were blotted onto a membrane and enzymatically digested after reduction and alkylation. The tryptic peptide mixture is analysed by MALDl-MS to achieve a peptide map or peptide mass fingerprint (PMF). The m/z information of the peptides is used to search the protein database, e.g., the Protein Identification Resource (PIR) database [112-114]. If the mass of just 4-6 tryptic peptides is accurately measured (between 0.1 and 0.01%), a useful database search can be performed. 

The first resources for computer modeling of protein structure are the nucleic acid and protein sequence databases (see Table 6.1), curated by the European Molecular Biology Laboratory (EMBL) in Europe, the National Center for Biotechnology Information (GenBank at the NCBl) in the United States, and the DNA Database of Japan (DDBJ) in Japan. These databases are accessible via the Internet, and most likely one s own scientific institution maintains a local version, which is updated through CD-ROMs released quarterly. Perhaps the predominant protein sequence database is SWISS-PROT. - Others include the nonredundant protein sequence database (OWL) and the protein identification resource database (PIR). ... 

Protein Identification Resource National Biomedical Research Foundation Georgetown University Medical Center 3900 Reservoir Road, N.W., Washington, DC 20007 DEC VAX... 

Protein and peptide sequences are stored in 1. the Protein Identification Resources [National Biomedical Research Foundation, 39W Reservoir Road, Washington, DC 200007, USA (pirmail gunbrf.bitnet) and 2. in SwissProt (European Bioinformatics Institute). 

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