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Protein expression integration with cloning

Since the procedure described here will yield clones with random integrations of the RNAi transgene, there will he varying levels of shRNA expression, due to position effect variegatioa It is difficult and unnecessary to quantitatively measure the levels of shRNA in each ES cell clone. The most definitive way to screen ES cell clones for knockdown is by Western analysis of equivalent amounts of protein lysate. This requires the availability of a good antibody that recognizes your protein of interest and that the gene is expressed in ES... [Pg.178]

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Expression clone

Expression cloning

Expression, proteins

Integral proteins

Proteins integrity

Proteins protein Integral

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