Protein Engineering of Trypsin-Like Proteases

Variants of trypsin with increased aminolysis rate in substrate-mimetic-mediated peptide synthesis have been obtained by mutating active site residues and residues in the SI subsite. A variant called trypsiligase was described by Bordusa and coworkers [97]. It carries the mutations D189K (reduces Arg and tolerates Tyr specificity in the 

15 DISCOVERY AND ENGINEERING OF ENZYMES FOR PEPTIDE SYNTHESIS AND ACTIVATION 

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