Protein engineering libraries

Second, the flow cytometric isolation of very rare clones from a library is technically demanding. For most protein engineering applications microorganisms are used as the expression host. Whereas the sorting of rare mammalian cells is widely practiced in cell biology research and in clinical medicine and is therefore highly advanced, the screening and isolation of microbial cells is still much more of an art (Davey and Kell, 1996). 

Lutz S, Ostermeier M, Benkovic SJ. Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides. Nucleic Acids Res. 2001 29 el6. 

Moore GL, Maranas CD. Computational challenges in combinatorial library design for protein engineering. AlCHE J. 2004 50 262-272. 

For example, see Y. Wei and M. H. Hecht. Enzyme-like proteins from an unselected library of designed amino acid sequences. Protein Engineering Design and Selection, 17 (2004), 67-75. 

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