Protein engineering catalytic efficiency improvement

With at least partial understanding of essential features of enzymatic catalysis has come the desire to improve, by the modification of existing enzymes through genetic or chemical means to alter their substrate specificity without loss of catalytic efficiency, the field of protein engineering. A unique feature in the antibody design is that the stereospecific catalysis can be sought for reactions not known to be enzyme catalyzed. 

Whereas cleavable linkers seem less relevant with respect to engineering functional interactions between P450 and redox partner(s), flexible and rigid hnkers have been used frequently to construct a variety of artificial fusion proteins with the aim to increase the efficiency of electron transfer and improve the catalytic efficiency of the P450 system. 

---