Protein Determination Using Amido Black

For instance, Brilliant-Orange is bound to basic amino acids (histidine, arginine and lysine), and it is therefore used for assaying proteins in corn. Amido Black is prefers for determining milk proteins. From the available commercial kits Pierce s microprotein rapid stat kit and Bio-Rad Protein Assay Kit can be shown as examples. Examples of protein analysers based on this principle are also given in Table 11. 

Amido black dye Two years later, Grassman and Hanning developed another organic dye to be used on filter paper after electrophoresis amido black stain. It has moderate sensitivity. Today, amido black dye is used for colorimetric determination of electroblotted proteins on PVDF (poly-vinylidene difluoride) and nitrocellulose membranes. 

Ability to bind colored dyes, such as Amido Black 10 B, Ponceau S, and Coomassie brilliant blue (CBB). This property is not only utilized to stain protein bands after electrophoresis but can also be used in spectrophotometric methods for total protein determination. There are two major problems the unequal affinities and binding capacities of individual... 

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