Protease assay using succinylated

A succinylated casein derivative that has nearly all its amines blocked can be used as a substrate in protease assays (Hatakeyama et al., 1992). As the casein is degraded by a protease, free amines are created from a-chain cleavage and release of a-amino groups. The creation of... 

Figure 23.11 The peptide substrate Succinyl-Ser-Leu-Asn-Tyr-Pro-lle-isobutylamide used to assay the effectiveness of inhibitors against HIV protease.

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