Properties of Fructosyltransferase from A. aculeatus

The substrate specificity of the fructosyltransferase from A. acukatus was estimated using a variety of di-, tri-, and tetrasaccharides at 100 g/1. The assay was 

The pure enzyme displayed the preference sucrose raffinose 1-kestose nystose (in a ratio 100 24 10 6). Other carbohydrates such as turanose, cellobiose, melibiose, leucrose, methyl-a-D-glucopyranoside, and stachyose were also tested but their activity was negligible. Thus, the enzyme only recognized sugars containing a sucrose moiety in their chemical structure. The trisaccharide raffinose is equivalent to a sucrose molecule substituted at the C-6 hydroxyl group, whereas 

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