Properties and application of immobilized enzyme

Immobilization may have some impact on enzymes and their surroundings. Immobilized enzyme will show different characteristics as compared to the enzyme solution. As to most of the enzymes, after the immobilization, their stability, longevity and shelf life will greatly increase, but their own activities show a certain decline, and the catalytic characteristics of the free enzyme are also different. When recognizing and mastering these rules of the immobilized enzyme, we can effectively guide the usage. 

Compared with the activity of the enzyme solution, the immobilized enzyme activity is generally declined. The reasons are many (1) The combination of the immobilized enzyme with insoluble carrier causes changes in the structure (2) The combination of the important amino acid residues of the enzyme active 

---