Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Propagating phage

To complete the library construction, the heteroduplex CCC-dsDNA must be introduced into an E. eoli host that contains an F episome to enable M13 bacteriophage infection and propagation. Phage-displayed library diversities are limited by methods for introducing DNA into E. eoli, with the most efficient method being high-voltage electroporation. [Pg.262]

Fig. 5. Generation of mutants using single-stranded DNA. After cloning the target gene into M13, the phage is propagated in the E. coll dut, ung strain of E. Fig. 5. Generation of mutants using single-stranded DNA. After cloning the target gene into M13, the phage is propagated in the E. coll dut, ung strain of E.
Goff SP, Berg P (1976), Construction of hybrid viruses containing SV40 and lambda phage DNA segments and their propagation in cultured monkey cells, Cell 9 695-705. [Pg.69]

See other pages where Propagating phage is mentioned: [Pg.264]    [Pg.171]    [Pg.675]    [Pg.675]    [Pg.264]    [Pg.171]    [Pg.675]    [Pg.675]    [Pg.233]    [Pg.247]    [Pg.360]    [Pg.360]    [Pg.403]    [Pg.401]    [Pg.63]    [Pg.64]    [Pg.256]    [Pg.233]    [Pg.247]    [Pg.1499]    [Pg.1499]    [Pg.1550]    [Pg.440]    [Pg.452]    [Pg.481]    [Pg.685]    [Pg.686]    [Pg.361]    [Pg.42]    [Pg.44]    [Pg.208]    [Pg.213]    [Pg.221]    [Pg.222]    [Pg.222]    [Pg.249]    [Pg.155]    [Pg.395]    [Pg.413]    [Pg.415]    [Pg.481]    [Pg.24]    [Pg.29]    [Pg.62]    [Pg.124]    [Pg.138]    [Pg.237]    [Pg.380]    [Pg.380]    [Pg.382]    [Pg.389]   
See also in sourсe #XX -- [ Pg.234 ]



SEARCH



Phage

© 2024 chempedia.info