Saliva progesterone

In general most steroids of clinical interest can be measured in saliva. For some steroids, such as cortisol, estriol, and progesterone, the measurement of the sahvary concentration appears to be a reliable indicator of the free concentration in plasma. For others (e.g., testosterone, 17-hydroxyprogesterone, estradiol, and aldosterone) the chnical usefulness of salivary measurements has not yet been fully established. 

Dyas J, Read GF, Guha-Maulik T, Hughes lA, Riad-Fahmy D. A rapid assay for 17a-OH progesterone in plasma, saliva and amniotic fluid using a magnetis-able solid-phase antiserum. Ann Clin Biochem 1984 21 417-24. 

Both RIA and enzyme immunoassay methodologies have been applied to the determination of progesterone in saliva. Although sample extraction procedures are often necessary to eliminate protein influences/ direct methods are favored in routine laboratories and have been described.Rigorous standardization of sample collection and preparation is important for obtaining meaningful results for cHnical purposes... 

Evans JJ. Progesterone in saliva does not parallel unbound progesterone in plasma. Clin Chem 1986 32 542-4. 

O Rorke A, Kane MM, Gosling JP, Tallon DF, Fottrell PF. Development and validation of a monoclonal antibody enzyme immunoassay for measuring progesterone in saliva. Clin Chem 1994 40 454-8. 

Schramm W, Smith RH, Craig PA, Pack SH, Kuo HH. Determination of free progesterone in an ultrafiltrate of saliva coUected in situ. Chn Chem 1990 36 1488-93. 

TaUon DF, Gosling JP, Bucldey PM, Dooley MM, Cleere WF, O Dwyer EM, et al. Direct solid-phase enzyme immunoassay of progesterone in saliva. Clin Chem 1984 30 1507-11. 

Most of the assays now carried out with isoluminol derivatives are solid-phase competitive assays (Fig. 2), in which the analyte to be determined competes with the labeled analyte for the available binding sites on antibodies that are immobilized on the solid phase. Thus, plastic microspheres have been used to immobilize antibodies for estriol (K12) or thyroxine (W9). Estradiol antibodies have been immobilized onto plastic tubes (K7) or beads (K16). In all of the above cases, the amount of immunoconjugated hormone label is inversely proportional to the amount of free hormone in the analytical sample. Unconjugated labels are removed by decantation or aspiration from the solid phase, and the specific, immunoconjugated labels are measured in a luminometer. This approach has been successfully applied to progesterone analyses in either serum (D6) or saliva (D5). A review of several separation-based assays with isoluminol analogs was presented a few years ago by Kohen et al. (K18). 

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