Product oils chromatographic separation data

Chromatographic Separation Data for Bitumen and Product Oils... 

The application of ultraviolet (UV) spectroscopy in the elucidation of the structure of terpenes and other natural products was extensively used by R.B. Woodward in the early forties of the last century. On the basis of his large collection of empirical data, he developed a series of rules (later called the Woodward rules), which could be applied to nding out the structures of new natural substances by correlations between the position of UV maximum absorption and the substitution pattern of a diene or an a,p-unsaturated ketone (Woodward, 1941). He was awarded the Nobel Prize in Chemistry in 1965. However, it was not until the introduction of chromatographic separation methods and nuclear magnetic resonance (NMI spectroscopy into organic chemistry that a lot of further structures of terpenes were elucidated. The almost exponential growth in our knowledge in that eld and other essential oil constituents is essentially due to the considerable advances in analytical methods in the course of the last half century. 

A great deal of work has been carried out on the TLC separation of various classes of synthetic dyes on untreated plates. Dyes from various sources, namely, foodstuffs (fruits, oils spices, alcoholic products), coated tablets, leather, fibers, cosmetics, etc., are first extracted and then applied to the layers. About 200 ml of developing solvent in a chromatographic development tank is then used to develop the chromatograms up to 10 cm. The data for the di fferent developing solvents and adsorbents (stationary phases) used for the separation of a variety of synthetic dyes, along with specific characteristics of the separation procedure, if any, are tabulated in Table 1. Details of specific separations follow. 

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