Procedure A Case Study

Since calibration is time consuming and is often viewed as nonproductive work, the decisions in developing a calibration program often 

TABLE I Summary of Calibration Tests and Acceptance Criteria 

HPLC module Test Procedure Acceptance criteria 

Pump Flow accuracy Run pump at 0.3 and 1.5 mL/min (65% acetonitrile/water) and collect 5 mL from detector into a volumetric flask. Measure time. 5% 

Column Temperature Check actual column oven 35 2°C 

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